Automated Analysis report for HPA_Gildersleeve_Sigma_HighComp evaluated at 1 ug/mL

List of Primary HPA_Gildersleeve_Sigma_HighComp Motifs

Primary Motif ID Primary Motif Minimal Structure Primary Motif Complete Structure Relative Binding Number of Glycans
A 1.00 22
B 0.65 3
C 0.57 2
D 0.06 17
0 0.00 186

Minimal and complete motif definitions match the same set of glycans defined in the array. Components found in the complete motif but excluded in the minimal motif are not tested by the array. Monosaccharide identities and subsitution intolerance may or may not have been tested by the array, they are retained from the complete motif for readability.

Boxplot of Primary Motifs

List of Fine-Specificity HPA_Gildersleeve_Sigma_HighComp Motifs

Motif ID Nearest Common Name (Accuracy%**) Motif Graphic Structure Relative Binding Number of Glycans P-Value***
A3 Terminal N-Actyl Galactosamine O-GalNAc (98%) 1.00 5 <0.001
A2 Blood Group A (97%) 0.99 4 <0.001
A6* Blood Group A (95%) 0.98 4 <0.001
A5* Blood Group A Type 1 (100%) 0.68 3 <0.001
A1 0.68 2 <0.001
A4 Blood Group A (96%) 0.51 2 <0.001
A0* Tn Antigen (100%) 0.48 2 <0.001
B0 0.53 3 <0.001
C0 Terminal alpha N-Acetyl Glucosamine (100%) 0.47 2 <0.001
D0* Terminal Type 2 LacDiNAc (100%) 0.11 3 0.909
D2 b-Series Ganglioside (100%) 0.11 2 0.989
D3 c-Series Ganglioside (100%) 0.08 2 0.999
D4* Terminal N-Actyl Galactosamine a-Series Ganglioside (99%) 0.01 5 1.000
D1 Terminal N-Actyl Galactosamine O-GlcNAc Core (97%) 0.00 2 1.000
D5 Terminal N-Actyl Galactosamine O-GlcNAc Core (97%) 0.00 3 1.000
0 Non-Binders (100%) 0.00 186 NA

Key:

See Symbol Nomenclature for Glycans (SNFG) for complete key: https://www.ncbi.nlm.nih.gov/glycans/snfg.html

*Motif indicates the remaining glycans not matched by motifs which are a subset. Motif definition needs to be taken in the context of the model.

**Accuracy describes the consistency between common-name definition of the motif and the formal, text-based definition of the motif, in terms of percent agreement in the glycans containing the two motifs. Common Name label definitions given here.

***P-Value refers to difference from Non-Binders with multiple testing correction (Dunnet’s Test)

Motifs with a red motif ID fail to show a logistic response to protein concentration in the range of concentrations analyzed. These motifs may be nonbinding motifs (motifs which define nonbinding exceptions) or simply fail to fit. Nonbinding motifs are determined based on concentration dependent response when available or the average binding of non-motif glycans otherwise.

Boxplot of Fine-Specificity Motifs

Figure 1. Glycan binding grouped by motif and motif family. Individual glycans are given as points on the plot.

Motif Intensity Map

Figure 2. Glycan intensity and motif distribution plot. The top half of the plot presents the observed glycan binding intensity of various glycans used in the array over their rank binding intensity; only the top glycans are shown. The second plot indicates the position of glycans containing the various motifs in the top plot with a yellow tick.

Motif Family Membership Map

*Motif indicates the remaining glycans not matched by motifs which are a subset. Motif definition needs to be taken in the context of the model.

Figure 3. Treemap of glycan binding grouped by motif and family structure. The model structure can be represented as nested boxes where box size is proportional to the number of glycans with the motif and color changes with change in average relative binding of glycans with the motif. Only three layers of data splitting are included here, though further splitting may be possible.

Detailed Model Breakdown

Motif Glycan Examples:

Motif ID Motif Minimal Graphic Motif Complete Graphic Highest Glycan Moderate Glycan Lowest Glycan
A1
A2
A3
A4
A5
A6
A0
B0
C0
D1
D2
D3
D4
D5
D0
0

Key:

All Concentration Plots:

Figure 4. Boxplots of glycan binding grouped by motif for each dataset in the model. Motifs are listed in ascending average binding intensity (for the selected concentration) and colored by family.

Model Structure:

*Motif matches the remaining glycans not matched by earlier motifs in the model.

Figure 5. Tree representation of the regression tree model trained on array data. Data flows through the tree (top-down) and is split by the various motifs. The motif used the split the data at each point has the id “family+split number” except when further split. In the case of futher splits the id of the motif used to split the data is denoted with an asterisk.


Curve Fitting:

Figure 6. Logistic curves fit to average motif binding. Curves are fit with as many parameters as possible given the data. All curves are based on the logistic curve with a fixed intercept of 0. Nonbinding motifs are excluded from the plot.

Motif Curve Parameter Scatterplots

Figure 7. Scatterplots of motif curve parameters. Plotting the asymptote of the curve and the slope of the curve over the log 10 Kd of the motif allows for critical assesment and comparison of motif curves. Note that the apparent Kd of the motif is the concentration at which the curve is half of the asymptote. A standard logistic slope of 1 is marked in a dashed line for reference. Nonbinding motifs are excluded from the plot. The highest affinity motifs which show the best concentration dependent response are in the top left of the top plot. Curves which have very low slopes may also indicate nonspecific binding while higher slopes may indicate higher order binding.

Motif ID Motif Graphic Structure Estimated Kd (ug/mL) Curve Asymptote Hill Slope
A1 0.538 34037.0 1.032
A2 0.388 46805.7 1.001
A3 0.441 43613.9 1.124
A4 0.759 33616.3 0.832
A5* 0.387 30757.0 1.051
A6* 0.451 42961.8 1.137
A0* 0.953 31937.0 0.847
B0 1.315 38541.7 0.763
C0 0.576 27532.0 0.846
D1 43.794 18015.6 1.214
D2 9.941 33873.2 0.950
D3 15.335 29594.0 0.857
D4* 16.074 11638.9 1.285
D5 0.037 41.1 0.000
D0* 2.498 12278.3 0.911

Motif Text Structures:

Motif ID Motif Graphic Motif Text
A1 <3f4f6f>GalNAcA1-6<1f2f3f4f>GalB
A2 <3f4f6f>GalNAcA1-3<4f6f>GalB1-4<1f6f>GlcNAcB
A3 <3f4f6f>GalNAcA1-3<4f6f>GalNAcB
A4 <3f4f6f>GalNAcA1-3<4f6f>GalB1-3<6f>GlcNAcB1-3<2f4f6f>GalB
A5* <3f4f6f>GalNAcA1-3<4f6f>GalB1-3<6f>GlcNAcB
A6* <3f4f6f>GalNAcA1-3<4f6f>GalB
A0* <3f4f6f>GalNAcA
B0 <3f4f6f>GalNAcB1-4<1f2f3f6f>GalB
C0 <3f4f6f>GlcNAcA
D1 <3f4f6f>GalNAcB1-4<2f6f>GalB1-4<1f3f6f>GlcNAcB
D2 <4f6f8f>Neu5AcA2-8<4f6f>Neu5AcA2-3(<3f4f6f>GalNAcB1-4)<2f6f>GalB
D3 <4f6f8f>Neu5AcA2-8<4f6f>Neu5AcA2-8<4f6f>Neu5AcA2-3(<3f4f6f>GalNAcB1-4)<2f6f>GalB
D4* <3f4f6f>GalNAcB1-4(<4f6f>Neu5AcA2-3)<2f6f>GalB
D5 <3f4f6f>GalNAcB1-3<2f4f6f>GalA
D0* <3f4f6f>GalNAcB
0 Non-Binders

Key: